1 August 2019 by Remco Bouckaert

The effective sample size (ESS) is an important diagnostic for MCMC analyses to determine whether the run converged. There is already information on this page, but here we discuss a few more things you can consider to increase the ESS.

## Increase BEAST performance

Often, you can increase speed of the chain by running it more efficiently, as outlined in the performance suggestions page. Essentially, you want to use the BEAGLE library, and use as many threads as help increase the number of MCMC samples per million states, which are reported on screen when BEAST runs.

## Reduce complexity of the model.

In general, you want to make sure the analysis runs fine with the simplest model first before adding more complexity to it, so initially

- Use HKY instead of GTR.
- If you have many partitions, do not estimate mutation rates for partitions.
- Use strict clock instead of relaxed clock
- Use the simplest tree prior, i.e., Yule or Coalescent with constant population size. Using a birth/death model when there is little signal for the death rate will results in everything mixing, except for the prior and posterior.

Once the analysis converges with these settings, you can change aspects of the model to the point where the chain keeps mixing and giving good ESSs.

## Check your model is valid

If your model is set up with unidentifiable parameters all of the above will not help – even if the model has identifiable parameters, in practice the signal in the data may be too weak to be able to estimate them

An example of actual unidentifiable parameters is when the substitution rates and clock rates are both estimated for all partitions (see this post for details on the different rates). This shows up in Tracer as low ESSs for the rate parameters, but reasonable ESSs for tree likelihoods.

An example of often hard to estimate parameters are the birth and death parameters of birth/death models. This shows up in Tracer as low ESSs for the prior and the posterior, but most other values mixing reasonably.

## Balance operator weights

If only a few parameters (like the kappa and base frequencies parameters for the HKY substitution model) have low ESSs compared to other entries in the trace log, this can be fixed by increasing the operator weight. You can do this in BEAUti in the operators panel – by default, the panel is not visible, but by going to the `View/Show Operators panel`

menu, you can make it appear.

Alternatively, you can directly edit the XML, go to the section with operators and change the weight:

## Use operators that move multiple parameters

In Tracer, you can use the `joint marginal`

panel to show correlation between the different parameters in your analysis. Here, we see how clock rate, tree heigh and population size are correlated:

If the correlation is strong enough, and operators only work on one parameter at the time, it may prevent mixing. An operator that helps such mixing is the up-down operator, which is a scale operator that moves various parameters at the same time. You can add operators to the XML file, or add parameters to existing operators to help mixing.

For the up-down operator, you should group all parameters with positive correlation (positive slope of elipse on Tracer, here tree height and population size) in the `up`

elements, and the negative correlated parameters (here the clock rate) in the `down`

element. The Up/Down operator has the following attributes:

- scaleFactor (double): magnitude factor used for scaling (required)
- up (state node that can be scaled, like real parameter or tree): zero or more items to scale upwards (optional)
- down (state node): zero or more items to scale downwards (optional)
- optimise (boolean): flag to indicate that the scale factor is automatically changed in order to achieve a good acceptance rate (optional, default: true)
- elementWise (boolean): flag to indicate that the scaling is applied to a random index in multivariate parameters (optional, default: false)
- upper (Double): Upper limit of scale factor (optional, default: 1.0)
- lower (Double): Lower limit of scale factor (optional, default: 0.0)
- weight (Double): weight with which this operator is selected (required)